A method for the determination of nucleotide sequence in polyribonucleotides.
نویسنده
چکیده
Our knowledge of the structure of ribonucleic acids (RNA) has advanced considerably in recent years. In the first place, the refinement of chromatographic techniques has made possible an accurate estimation of the purine and pyrimidine composition (Vischer & Chargaff, 1948; Markham & Smith, 1949; Carter, 1950; Markham & Smith, 1951). Secondly, with the aid of both chemical and enzymic methods it has been shown that the constituent nucleosides inRNA are linked together as in I,
منابع مشابه
Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosides.
A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide dialdehydes generated by treatment of polyribonucleotides with alkaline phosphatase and excess periodate at pH 8 (borate buffer; no primary amine present in the reaction mixture). While neither phosphatase nor periodate possess any intrinsic exonucleas...
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عنوان ژورنال:
- The Biochemical journal
دوره 58 3 شماره
صفحات -
تاریخ انتشار 1954